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Key Documents

GE17-1357-01

HiTrap® Butyl Fast Flow

Cytiva 17-1357-01, pack of 5 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

butyl

儲存期限

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包裝

pack of 5 × 1 mL

製造商／商標名

Cytiva 17-1357-01

參數

<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

4% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

2-14

工作範圍

3-13

適合性

suitable for bioprocess medium

相关类别

一般說明

HiTrap® Butyl FF are prepacked Butyl Sepharose™ 4 Fast FLow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

特點和優勢

  • Provide convenient and fast small-scale Protein purifications.
  • Butyl Sepharose™ 4 Fast FLow is a well-established, standard aliphatic HIC medium for capture and intermediate purification.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
  • High flow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

儲存和穩定性

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

实验方案

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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