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G3776

Sigma-Aldrich

鸟苷5′-三磷酸 钠盐 溶液

HPLC purified, aqueous solution for RNA polymerase transcription

别名:

GTP

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About This Item

CAS号:
36051-31-7
MDL號碼:
MFCD00077781
分類程式碼代碼:
41106305
PubChem物質ID:
24895138
NACRES:
NA.52

生物源

Porcine brain
bacterial (Corynebacterium)
yeast

品質等級

300

化驗

≥95% (HPLC)

形狀

liquid

濃度

100 mM in H2O (adjusted to pH 7)

顏色

colorless

異物活動

DNase, RNase and Nickase, none detected

運輸包裝

dry ice

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1

InChI 密鑰

KZRMTEVIDYXWQW-CYCLDIHTSA-K

基因資訊

human ... HRAS(3265)

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應用

5′-三磷酸鸟苷钠盐溶液已用于：

  • 在转导蛋白的纯化过程中重构转导蛋白。
  • 作为 3.3X 负载缓冲液的组分，组装 32P-miR-31—3xFLAG-AGO2 复合物
  • 作为核苷酸底物混合物的组分，以评估 MutT 同源物 1（MTH1）对甲基化核苷三磷酸盐的水解活性
  • DNA 依赖性 RNA 聚合酶转录

生化／生理作用

5′-三磷酸鸟苷（GTP）占总核苷酸三磷酸池的近 25%，是一种核苷酸阴离子。它参与多种生物功能，如通过激活 GTP 结合蛋白、RNA 和蛋白质合成的细胞信号传导，以及产生二级信使环鸟苷一磷酸（cGMP）。GTP 还参与了严格的信号素的生产。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type N95 (US)

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCJ3892
SLCB3545
SLBW7401
SLBP8495V
SLBL7597V

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Sammons J and Gross AK
Methods in Molecular Biology, 884, 167-181 (2012)
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Redox report : communications in free radical research, 14(2), 82-92 (2009-04-25)
Levels of oxidized guanosine base in DNA have become a hallmark biomarker in assessing oxidative stress implicated in a variety of disease and toxin-induced states. However, there is evidence that the guanosine in the nucleotide triphosphate pool (GTP) is more
Jonathan A Winger et al.
Biochemistry, 44(10), 4083-4090 (2005-03-09)
The catalytic domains (alpha(cat) and beta(cat)) of alpha1beta1 soluble guanylate cyclase (sGC) were expressed in Escherichia coli and purified to homogeneity. alpha(cat), beta(cat), and the alpha(cat)beta(cat) heterodimeric complex were characterized by analytical gel filtration and circular dichroism spectroscopy, and activity
P Barbier et al.
Biochemistry, 37(2), 758-768 (1998-02-21)
NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active
S A Cohn et al.
The Journal of biological chemistry, 264(8), 4290-4297 (1989-03-15)
We have analyzed the effects of various substrates and inhibitors on the rates of microtubule (MT) motility induced by sea urchin egg kinesin using real-time computer analysis and video-enhanced light microscopy. In the presence of magnesium, 10 mM concentrations of
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