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形狀
liquid
用途
200 uses (0.1 mg size)
40 uses (20 μg size)
適合性
suitable for electrophoresis (DNA)
儲存溫度
−20°C
相关类别
一般說明
Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
應用
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.
成分
Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
其他說明
Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.
準備報告
Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen
Mrazek J and Kopecny J
Folia Microbiologica, 46(1), 63-65 (2001)
商品
We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.
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