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應用
开发用于免疫组织学和免疫印迹,作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物,因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水,封片于树脂介质中,并储存以备将来引用。SIGMAFAST™ DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中
重構
将每片片剂溶于 1 mL 去离子水中,得到含 DAB 和过氧化氢尿素的即用型缓冲液。
法律資訊
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
5.1B - Oxidizing hazardous materials
閃點(°F)
53.6 °F - closed cup
閃點(°C)
12 °C - closed cup
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商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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