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一般說明
使用含金属强化剂的SIGMA FAST™ DAB可制备用于检测过氧化物酶活性的活性底物溶液,无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。
應用
含金属强化剂的SIGMAFAST™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节(DRG)神经元和前十字韧带(ACL)细胞中胶原蛋白的免疫组织化学染色。
通过添加CoCl2可以增强DAB反应,并产生更强的耐酒精色斑。
重構
将每个片剂组合溶于5 mL去离子水中,得到含有DAB和金属强化剂的即用型缓冲溶液。
其他說明
法律資訊
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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