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等級
for molecular biology
形狀
solution
分子量
31.5 × 103 kDa
48 kb
濃度
556 μg/mL
適合性
suitable for substrate for restriction endonucleases
運輸包裝
dry ice
儲存溫度
−20°C
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一般說明
Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This methylated lambda DNA is not completely digested by Bcl I, Cla I, Mbo I, Mbo II, Taq I or Xba I whereas non-methylated lambda DNA(product number D3654) is only partially cleaved.
特異性
独特的限制位点：Apa I、Nae I、Nar I、Nhe I、PaeR7 I、SnaB I、Xba I 和 Xho I。甲基化 lambda 被 Bcl I、Cla I、Mbo I、Mbo II、Taq I 和 Xba I 部分切割。
應用
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 has been used:
- in magnetic tweezers measurements
- in the preparation of 35S-labeled DNA
- in the preparation of coupled standards (DNA + RNA)
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used to study the attractive forces between condensed DNA double helix by combining single-molecule magnetic tweezers and osmotic stress. 35S-labeled Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 was also used to standardize a method of measuring dissolved DNA in seawater.
λ 噬菌体有一个二十面体头和一个长尾，末端为单根纤维。在 5′ 末端的两端是互补的 12 个核苷酸的单链序列，它们有助于 DNA 的粘性末端（cos 区）。噬菌体的尾部锁定在宿主外膜受体上，并将噬菌体 DNA 注入细胞。噬菌体将大肠杆菌转化为溶原性状态，其中噬菌体功能受到抑制，并且噬菌体基因组可能长时间保持休眠（原噬菌体）。这种特性在携带负责 CI 表达的 CII 和 CIII 基因的噬菌体中可见。CI 基因中具有 CI 突变的噬菌体能够在规定的温度下保持溶原状态。
用 λ C1857 菌株感染大肠杆菌菌株 W3110 会产生大肠杆菌溶原菌培养物。通过用 pH 8.0 的高盐缓冲液裂解，噬菌体从大肠杆菌细胞沉淀中释放出来。 将粗混合物通过一系列酶促步骤、多个铯梯度，然后用 1 mM Tris-HCl、pH 8.0 和 1 mM 氯化镁对噬菌体 DNA 进行透析。最后用苯酚-氯仿溶液提取 DNA。
用 λ C1857 菌株感染大肠杆菌菌株 W3110 会产生大肠杆菌溶原菌培养物。通过用 pH 8.0 的高盐缓冲液裂解，噬菌体从大肠杆菌细胞沉淀中释放出来。 将粗混合物通过一系列酶促步骤、多个铯梯度，然后用 1 mM Tris-HCl、pH 8.0 和 1 mM 氯化镁对噬菌体 DNA 进行透析。最后用苯酚-氯仿溶液提取 DNA。
單位定義
一外径（A260） 是大约 50 μg DNA
外觀
DNA is supplied in a solution of 10 mM Tris-HCl (pH 8.0), with 1mM EDTA.
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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Limnology and Oceanography: Methods, 2, 248-255 (2004)
A novel method for the measurement of dissolved deoxyribonucleic acid in seawater
Brum J R, et al.
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Brian A Todd et al.
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By combining single-molecule magnetic tweezers and osmotic stress on DNA assemblies, we separate attractive and repulsive components of the total intermolecular interaction between multivalent cation condensed DNA. Based on measurements of several different cations, we identify two invariant properties of
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