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Merck

D1946

Sigma-Aldrich

二氨基荧光素-FM 二乙酸酯

≥98% (HPLC)

别名:

4-氨基-5-(N-甲氨基)-3′,6'-双(乙酰氧基)-2′,7'-二氟-螺[ 异苯并呋喃-1 (3H) ,9'-[9H]黄原酮]-3-酮, DAF-FM DA

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About This Item

经验公式(希尔记法):
C25H18F2N2O7
分子量:
496.42
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.77

品質等級

化驗

≥98% (HPLC)

形狀

film

儲存條件

desiccated

顏色

colorless to yellow

螢光

λex 500 nm; λem 515 nm in DMSO

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI 密鑰

BEVHTVRRVVEMEF-UHFFFAOYSA-N

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應用

DAF-FM DA 已被用于测量肝组织匀浆中的一氧化氮 (NO) 水平。它还被用于检测人脐静脉内皮细胞 (HUVEC) 的 NO 染色。

生化/生理作用

DAF-FM DA 是一种用于检测一氧化氮 (NO) 的细胞渗透性荧光探针。DAF-2 是最初的化合物,应用广泛,但 DAF-FM 和 DAF-FM DA 与 DAF-2 相比对一氧化氮 (NO) 更敏感,更耐光且pH 敏感性较低。DAF-FM DA 能很好地渗透到活细胞中,并通过胞浆酯酶迅速转化为水溶性 DAF-FM。

特點和優勢

该化合物在受体分类和信号转导手册的一氧化氮合酶页面上有重点介绍。想要浏览手册的其他页面, 请单击此处

注意

吸湿

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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