251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• 别名: DAF-2, 4,5-Diaminofluorescein
• 经验公式（希尔记法）: C₂₀H₁₄N₂O₅
• CAS号: 205391-01-1
• 分子量: 362.34
• MDL號碼: MFCD05865100
• 分類程式碼代碼: 12171500
• NACRES: NA.25

属性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: solution
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: pale yellow
• 螢光: λ_ex ~495 nm; λ_em ~515 nm
• 儲存溫度: −20°C
• SMILES 字串: Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• InChI 密鑰: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

说明

一般說明

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

生化／生理作用

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

警告

Toxicity: Irritant (B)

外觀

A 5 mM (1 mg in 550 µl) solution in DMSO.

重構

Following initial thaw, aliquot and freeze (-20°C).

其他說明

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

法律資訊

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: 188.6 °F - closed cup - (Dimethylsulfoxide)
• 閃點（°C）: 87 °C - closed cup - (Dimethylsulfoxide)