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Merck

D0184

Sigma-Aldrich

2′-脱氧尿苷 5′-三磷酸 钠盐 溶液

100 mM in H2O

别名:

dUTP 钠盐

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About This Item

经验公式(希尔记法):
C9H15N2O14P3 · xNa+
CAS号:
分子量:
468.14 (free acid basis)
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.52

化驗

≥99%

形狀

liquid

濃度

100 mM in H2O

顏色

colorless

異物活動

DNase, RNase, none detected

運輸包裝

dry ice

儲存溫度

−20°C

SMILES 字串

[Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O

InChI

1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;

InChI 密鑰

FVFWWPFKJNTHIQ-UHFFFAOYSA-N

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相关类别

一般說明

2′-Deoxyuridine 5′-triphosphate (dUTP) is incorporated into DNA during DNA synthesis. It is one of the main intermediate during pyrimidine nucleotide biosynthesis. It is one of the sources of uracil in DNA. Deoxyuridine 5′-triphosphate nucleotidohydrolase suppresses the incorporation of dUTP into DNA. It converts dUTP to 2′-deoxyuridine 5′-monophosphate (dUMP).

應用

2′-Deoxyuridine 5′-triphosphate sodium salt solution has been used in the mouse genomic DNA amplification by polymerase chain reaction (PCR). It is also suitable for conjunction with uracil DNA glycosylase to prevent carryover contamination in PCR. The dUTP is substituted for dTTP in the PCR reaction.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Abundant non-canonical dUTP found in primary human macrophages drives its frequent incorporation by HIV-1 reverse transcriptase.
Kennedy EM
The Journal of Biological Chemistry, 286(28), 25047-25055 (2011)
dUTP incorporation into genomic DNA is linked to transcription in yeast.
Kim N
Nature, 459(7250), 1150-1153 (2009)
M C Longo et al.
Gene, 93(1), 125-128 (1990-09-01)
Polymerase chain reactions (PCRs) synthesize abundant amplification products. Contamination of new PCRs with trace amounts of these products, called carry-over contamination, yields false positive results. Carry-over contamination from some previous PCR can be a significant problem, due both to the
DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.
T Lindahl et al.
The Journal of biological chemistry, 252(10), 3286-3294 (1977-05-25)

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