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Merck
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Key Documents

CMC0012

Sigma-Aldrich

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.85

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

特點和優勢

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA

成分

  • XLDNA SIG10 electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


儲存類別代碼

10 - Combustible liquids


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