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Merck
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文件

CG200

Sigma-Aldrich

cGMP 酶免试剂盒，直接

sufficient for 96 assays

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 96 assays

品質等級

200

技術

ELISA: suitable

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... PRKG1(5592) , PRKG2(5593)

應用

定量分析组织和细胞培养物中总cGMP的非放射性、竞争性免疫检测方法。缓冲液含有0.1N HCl，促进细胞裂解、抑制内源性磷酸二酯酶并稳定环核苷酸。该试剂盒使用cGMP多克隆抗体，后者能够竞争性结合cGMP或共价连接碱性磷酸酶分子的cGMP。首先在抗兔IgG抗体包被的96孔板中向孔中加入底物。反应产生有色终产物，然后在多孔板酶标仪上读取405nm处的吸光度。对应颜色的荧光强度与孔中cGMP的浓度成反比。
有关详情，请参阅随附 方案

仅试剂盒组分

产品编号
说明
  • 0.1 M Hydrochloride Acid 1 x 30
  • Acetic Anhydride 1 x 1
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • Neutralizing Reagent 1 x 6
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
查看所有结果 (12)

訊號詞

Danger

危險分類

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

標靶器官

Respiratory system

安全危害

EUH071

儲存類別代碼

3 - Flammable liquids

閃點（°F）

120.2 °F

閃點（°C）

49 °C

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Lance M Wheeler et al.
Nature communications, 8(1), 1722-1722 (2017-11-25)
Materials with switchable absorption properties have been widely used for smart window applications to reduce energy consumption and enhance occupant comfort in buildings. In this work, we combine the benefits of smart windows with energy conversion by producing a photovoltaic
Yuichiro Kunai et al.
Journal of the American Chemical Society, 139(43), 15328-15336 (2017-10-08)
The concept of electrical energy generation based on asymmetric chemical doping of single-walled carbon nanotube (SWNT) papers is presented. We explore 27 small, organic, electron-acceptor molecules that are shown to tune the output open-circuit voltage (V
Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)

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