HF1

94101908

• 分類程式碼代碼: 41106514

属性

• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Modal no. 100
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat hepatoma Hybrid [H5xFao]

細胞系描述

HF1 is a clone of hybrid cells resulting from the fusion of H5 and Fao. Expression of the majority of hepatic functions are extinguished. The cells contain a number of chromosomes corresponding to the sum of the parental numbers. HF1 variant which lacks the complete set of tissue-specific functions, appears to be a good candidate for regulatory mutants which control the orderly temporal expression of tissue specific functions during development.

應用

Analysis of gene expression by molecular techniques.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 9x1,000 to 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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