BE(2)-M17

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95011816, human nerve, Fusiform

• 分類程式碼代碼: 41106514

属性

• 生物源: human nerve
• 包裝: tube of 5 μg 95011816-DNA-5UG; pkg of vial of cells 95011816-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fusiform
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian neuroblastoma

細胞系描述

The cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.

應用

Study of neuroblastoma

培養基

EMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm² using 0.1% trypsin or PBS only; 5% CO₂; 37°C.

其他說明

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