MFE-280

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98050131, human endometrium, Epithelial

• 别名: MFE280 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human endometrium
• 包裝: tube of 5 μg 98050131-DNA-5UG; pkg of vial of cells 98050131-1VL
• 增長模式: Adherent
• 染色體組型: Near diploid
• 形態學: Epithelial
• 產品: Placental protein 14
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human caucasian endometrial adenocarcinoma

細胞系描述

MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.

應用

Tumour biology studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17

培養基

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

其他說明

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