KYSE-410

94072023, human esophagus, Epitheloid

• 别名: KYSE0410 Cells, KYSE410 Cells
• 分類程式碼代碼: 41106514

属性

• product name: KYSE-410, 94072023
• 生物源: human esophagus
• 增長模式: Adherent
• 染色體組型: Hypotetraploid with 4% polyploidy
• 形態學: Epitheloid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human oesophageal squamous cell carcinoma

細胞系描述

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

培養基

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

例行更新培養

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C. Doubling time ~45h

其他說明

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