L-2

90112811, rat lung, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: L-2, 90112811
• 生物源: rat lung
• 增長模式: Adherent
• 染色體組型: Modal no. 77
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat lung

細胞系描述

Derived from normal rat lung tissue. The cells possess elevated levels of specific enzymes involved in the synthesis of phosphatidyl choline. Osmiophilic lamellar inclusions are observed in cell pellets from mass culture.

培養基

Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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