LL47 (MaDo)

90102538, human lung, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• product name: LL47 (MaDo), 90102538
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: 2n = 46; diploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human diploid lung

細胞系描述

Derived from normal lung tissue obtained from a 16 year old male with osteogenic sarcoma. Ethnicity: Black.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16

培養基

Coon′s modified Ham′s F12 + Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. A further 15 population doublings expected.

其他說明

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