K-BALB (K-234)

89092101, mouse embryo, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: K-BALB (K-234), 89092101
• 生物源: mouse embryo
• 增長模式: Adherent
• 染色體組型: 2n=40
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse A31 embryo Kirsten sarcoma, transformed

細胞系描述

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

其他說明

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