GH1

89072610, rat brain, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: GH1, 89072610
• 生物源: rat brain
• 增長模式: Semi-adherent
• 染色體組型: Hypodiploid, modal no. 40.
• 形態學: Epithelial
• 產品: Rat growth hormone, lactogenic hormones.
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Wistar-Furth pituitary tumour

細胞系描述

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

應用

Hormone studies

培養基

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

其他說明

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