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Key Documents

AtT20

87021902, mouse brain, Small round cells growing as large aggregates

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About This Item

分類程式碼代碼:
41106514

product name

AtT20, 87021902

生物源

mouse brain

增長模式

Aggregates in suspension

染色體組型

Diploid

形態學

Small round cells growing as large aggregates

產品

Adrenocorticotropic hormone (ACTH)

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse LAF1 pituitary gland tumour

細胞系描述

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

應用

Hormone studies

培養基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

例行更新培養

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

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