BRIN-BD11

10033003, from rat pancreas(islets)

• 别名: BRIN
• 分類程式碼代碼: 41106514

属性

• 生物源: rat pancreas (islets)
• 增長模式: Not specified
• 染色體組型: Modal chromosome number 58 over 50 passages
• 形態學: Epitheloid
• 產品: BRIN-BD11 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BD11 cells express the GLUT2 glucose transporter.
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

说明

細胞系來源

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

細胞系描述

BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.

DNA分析

Not specified

培養基

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

例行更新培養

Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 20hrs. At confluence 10⁵ cells/cm² can be expected.

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.