推荐产品
生物源
bovine spleen
品質等級
種類
Type X
化驗
>25% protein (biuret)
形狀
lyophilized powder
比活性
≥5 units/mg protein
成份
Protein, ≥25% biuret
製造商／商標名
Sigma-Aldrich
儲存條件
OK to freeze (Unstable. Keep frozen)
濃度
≥5 unit/mg protein
技術
activity assay: suitable
適合性
suitable for molecular biology
應用
life science and biopharma
運輸包裝
dry ice
儲存溫度
−20°C
基因資訊
cow ... CTSC(352958)
一般說明
Research Area: Cell Signaling
Dipeptidyl peptidase I (DPPI), also known as cathepsin C, is an abundant lysosomal cysteine protease from the papain superfamily with a molecular weight of approximately 200 kDa. It is widely expressed in a variety of mammalian tissues, with the highest levels found in the lungs, kidneys, liver, and spleen, and relatively lower levels in the brain.
DPPI is the only member of its family that is functional as a tetramer, consisting of four identical subunits, each composed of an N-terminal fragment, a heavy chain, and a light chain. It is identified as one of the multifaceted protease-processing machines, having been shown to function beyond its role as a non-specific lysosomal protease.
Dipeptidyl peptidase I (DPPI), also known as cathepsin C, is an abundant lysosomal cysteine protease from the papain superfamily with a molecular weight of approximately 200 kDa. It is widely expressed in a variety of mammalian tissues, with the highest levels found in the lungs, kidneys, liver, and spleen, and relatively lower levels in the brain.
DPPI is the only member of its family that is functional as a tetramer, consisting of four identical subunits, each composed of an N-terminal fragment, a heavy chain, and a light chain. It is identified as one of the multifaceted protease-processing machines, having been shown to function beyond its role as a non-specific lysosomal protease.
應用
来自Sigma的酶已被用于激活大肠杆菌颗粒酶k（Gzmk）前体。颗粒酶是颗粒形式储存的淋巴细胞丝氨酸蛋白酶，其与T和自然杀伤细胞介导的细胞毒性防御反应有关。
组织蛋白酶C已被用于一项研究中，其证明了蛋白质组学方法的潜力，该方法鉴定出绒毛外滋养层细胞表达的新型蛋白质并揭示导致妊娠疾病的机制。组织蛋白酶C也已被用于评估可生物降解的热凝胶的研究中。
Cathepsin C from bovine spleen has been used for the in vitro enzyme activity assays. It has also been used as a digestion enzyme for in vitro myelin oligodendrocyte glycoprotein (MOG) digestion.
生化／生理作用
组织蛋白酶C是一种二肽基氨基肽酶，可以用其未取代的N端从肽链上顺序去除二肽。该酶表现出对甘氨酸和脯氨酸作为N-末端氨基酸的偏好。具有N-末端赖氨酰基或精氨酰基残基，或倒数第二个氨基酸为脯氨酰基残基的底物不受此酶的影响。肽链内切酶活性需要卤离子和巯基活化剂的存在。
Cathepsin C (Cat C) serves as the physiological activator of groups of serine proteases within immune and inflammatory cells, playing a crucial role in the defense mechanisms of an organism. It may play a role in chronic airway diseases such as asthma. Cat C also acts as a protease link between inflammation and thrombosis.
Cat C participates in neutrophil recruitment and production of chemokines and cytokines in many inflammatory diseases. Cathepsin C plays a crucial role as an essential enzyme in activating granule serine proteases in cytotoxic T lymphocytes, natural killer cells (granzymes A and B), mast cells (chymase and tryptase), and neutrophils (cathepsin G, proteinase 3, and elastase).
Cat C participates in neutrophil recruitment and production of chemokines and cytokines in many inflammatory diseases. Cathepsin C plays a crucial role as an essential enzyme in activating granule serine proteases in cytotoxic T lymphocytes, natural killer cells (granzymes A and B), mast cells (chymase and tryptase), and neutrophils (cathepsin G, proteinase 3, and elastase).
注意
不稳定。保持冷冻。
單位定義
在pH 6.8、37°C条件下，使用DL-苯丙氨酸异羟肟酸为标准品，一个单位每分钟可从Gly-Phe-NH2和羟胺中产生1 μmole Gly-Phe-NHOH 。组织蛋白酶C除了具有水解特性外，还可催化二肽酰胺的聚合。
外觀
从1 M氯化钠溶液中冻干获得。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
The specificity of dipeptidyl transferase.
I M Voynick et al.
Biochemistry, 7(1), 40-44 (1968-01-01)
H Lindley
The Biochemical journal, 126(3), 683-685 (1972-02-01)
1. The characterization of cathepsin C as an aminodipeptidase has been confirmed. 2. An extra limitation on its specificity, namely that peptides involving proline as the third residue are immune to the enzyme, has been found. 3. A novel technique
High immunoglobulin E in a Chinese Papillon-Lefèvre syndrome patient with novel compound mutations of cathepsin C.
Xuan Wen et al.
The Journal of dermatology, 39(7), 664-665 (2012-01-10)
Case of rippled-pattern sebaceoma with clinically yellowish surface and histopathological paucity of lipid-containing neoplastic cells.
Yoshio Kawakami et al.
The Journal of dermatology, 39(7), 644-646 (2011-11-15)
Mayumi Ueta et al.
Japanese journal of ophthalmology, 55(4), 405-410 (2011-05-28)
We previously reported that human conjunctival epithelial cells expressed functioning interleukin-4 receptor α (IL-4Rα). In this study, we investigated whether human corneal epithelial cells also express functioning IL-4Rα. The presence of IL-4Rα mRNA and protein in human corneal epithelium was
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