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生物源
bovine heart
品質等級
描述
Contains primarily isozyme MM
種類
Type III
形狀
salt-free, lyophilized powder
比活性
≥30 units/mg protein
分子量
80 kDa
儲存溫度
−20°C
一般說明
Research area: Cell Signaling
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).
應用
来自牛心脏的肌酸磷酸激酶已被用于研究缺血心脏是否刺激内皮细胞生长。来自牛心脏的肌酸磷酸激酶也被用于评估高水平但无毒的铜和硒膳食摄入对小牛代谢的影响。
来自牛心脏的肌酸磷酸激酶已被用于：
- 加入反应混合物中，用于向分离线粒体中引入体外合成的野生型FLAG/MYC标记的LYR基序蛋白7（LYRM7-F / M）或丙氨酸取代LFK三肽（LFK-AAA）突变体
- 自动泛素化测定
- 制备预混缓冲液，用于腺苷酸环化酶活性测定
- 制备用体外核组装和分离的E-mix
生化／生理作用
肌酸激酶在具有间歇性高且波动性能量需求的细胞能量代谢中具有关键作用。这类细胞包括心脏或骨骼肌细胞以及脑和视网膜的神经组织等。该酶催化磷酰基从磷酸肌醇向ADP的可逆转移，以产生ATP。已发现该蛋白质的分子量约为80,000Da。它由2个亚基组成，每个亚基的分子量为40,000±2000。较轻的亚基含量较高。
注意
建议在反应缓冲液中使用0.1％白蛋白，以避免因稀释而失活。
單位定義
一个酶活性单位是指在pH 7.4、30°C条件下，每分钟将1.0 μmole磷酸盐从磷酸肌酸转移至ADP所需的酶量。
分析報告
通过缩二脲法测定的蛋白质。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Isolation, purification and characterization of creatine kinase from bovine cardiac muscle.
O S Herasymowych et al.
Biochimica et biophysica acta, 534(1), 38-47 (1978-05-24)
M S Fehrs et al.
Journal of dairy science, 64(8), 1700-1706 (1981-08-01)
Sixteen male Holstein calves in a 2 X 2 factorial design with four animals per treatment were fed 0 and 100 ppm supplemental copper from copper carbonate and 0 and 1 ppm added selenium from sodium selenite for 15 days
A C Galloway et al.
Surgery, 96(2), 435-439 (1984-08-01)
The development of myocardial ischemia is known to elicit the formation and enlargement of collateral vessels. The stimulus for these events is unknown. We have investigated the possibility that cardiac tissue releases a factor that can stimulate endothelial cell proliferation.
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Gabrielsen M, et al.
Molecular Cell, 68(2), 456-470 (2017)
Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for western blotting and scanning electron microscopy (SEM)
Allen TD, et al.
Nature Protocols, 2(5), 1173-1173 (2007)
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