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生物源
Escherichia coli
品質等級
重組細胞
expressed in E. coli overproducing strain
化驗
>95% (SDS-PAGE)
形狀
lyophilized powder
技術
electron microscopy: suitable
mass spectrometry (MS): suitable
適合性
passes test (Refolding (w/GroES))
UniProt登錄號
儲存溫度
2-8°C
基因資訊
human ... HSPD1(3329)
一般說明
研究领域:细胞信号传导
伴侣蛋白60(GroEL)和伴侣蛋白10(GroES)属于在原核生物和真核细胞器中普遍存在的热休克分子伴侣家族。
伴侣蛋白60(GroEL)和伴侣蛋白10(GroES)属于在原核生物和真核细胞器中普遍存在的热休克分子伴侣家族。
應用
来自大肠埃希菌的伴侣蛋白60已用于:
- 质谱
- 作为测试颗粒分布的对照品用于冷冻电子显微镜成像
- 作为红外光谱测量的标准品
- 以差分迁移率分析(DMA)和冷冻电子显微镜(cryo-EM)进行的适用性评估和表征研究中的蛋白质样品。
生化/生理作用
除了促进蛋白质折叠的作用外,伴侣蛋白60(Cpn60)可作为胞外信号传导蛋白,其功能类似于某些促炎性细胞因子。此外,Cpn60有助于抑制细胞分化初期的脂质蓄积和脂肪生成,从而具有抗肥胖作用。
包裝
包装尺寸基于蛋白质含量。
外觀
含有Tris缓冲盐、氯化钾、氯化镁、二硫苏糖醇和海藻糖作为稳定剂的冻干粉末。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Infrared irradiation in the collision cell of a hybrid tandem quadrupole/time-of-flight mass spectrometer for declustering and cleaning of nanoelectrosprayed protein complex ions
Analytical Chemistry, 82(23), 9878-9884 (2010)
Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
Scientific Reports, 4(2), 7084-7084 (2014)
Separating and visualising protein assemblies by means of preparative mass spectrometry and microscopy
Journal of Structural Biology, 172(2), 161-168 (2010)
Methods in molecular biology (Clifton, N.J.), 2247, 173-191 (2020-12-11)
By maintaining intact multi-protein complexes in the gas-phase, native mass spectrometry provides their molecular weight with very good accuracy compared to other methods (typically native PAGE or SEC-MALS) (Marcoux and Robinson, Structure 21:1541-1550, 2013). Besides, heterogeneous samples, in terms of
Nature protocols, 15(3), 1132-1157 (2020-02-02)
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer
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