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Merck

C5499

Sigma-Aldrich

细胞色素c氧化酶 来源于牛心脏

greener alternative

5 mg protein/mL

别名:

EC 1.9.3.1, 亚铁细胞色素c:氧气氧化还原酶,复合物IV

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

bovine heart

品質等級

形狀

buffered aqueous solution

比活性

≥20 units/mg protein

分子量

300-350 kDa by gel filtration

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

濃度

5 mg protein/mL

環保替代類別

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。对于生物燃料电池研究,本品已根据提高能效和防止废物原则进行了改进。有关详细信息,请参阅《Biofiles》的文章

應用

Cytochrome c Oxidase (CcO) from bovine heart has been used:
  • to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines
  • in a histochemical assay to measure CcO activity in tissue sections
  • to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

生化/生理作用

细胞色素c氧化酶是全部动物,植物,酵母菌和某些细菌有氧代谢中高氧亲和力的主要末端氧化酶。 它存在于高度发育的细胞的线粒体和细菌的细胞质膜中。细胞色素c氧化酶催化电子从细胞色素c转移到O2。这种电子转移过程会在整个膜上产生质子梯度,进而驱动ATP的产生。这种酶在通过将通过细胞色素链的电子传输与氧化磷酸化过程耦合为细胞提供能量方面是独特的。

單位定義

在25℃,pH值6.0下,一单位细胞色素c氧化酶每分钟将氧化1.0μ摩尔亚铁细胞色素c。

外觀

以溶于 25 mM Tris HCl缓冲液, pH 7.8,5 mM EDTA和 39 mM 正十二烷基β·-D-麦芽糖苷的溶液形式提供

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Claudia R Oliva et al.
The Journal of biological chemistry, 291(46), 24188-24199 (2016-09-30)
The enzyme cytochrome c oxidase (CcO) or complex IV (EC 1.9.3.1) is a large transmembrane protein complex that serves as the last enzyme in the respiratory electron transport chain of eukaryotic mitochondria. CcO promotes the switch from glycolytic to oxidative
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