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C3131

Sigma-Aldrich

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

别名:

CYC, CytC

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About This Item

CAS号:
9007-43-6
EC號碼:
232-700-9
MDL號碼:
MFCD00130890
分類程式碼代碼:
12352202
NACRES:
NA.61

生物源

bovine heart

品質等級

200

化驗

≥95% based on Mol. Wt. 12,327 basis

形狀

powder

分子量

12327 Da

儲存條件

(Tightly closed Dry)

技術

cell culture | mammalian: suitable

雜質

0.40-0.50% Iron (anhydrous)

溶解度

water: 10 mg/mL, dark red-brown

UniProt登錄號

P62894

儲存溫度

−20°C

基因資訊

cow ... CYC1(512500)

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一般說明

研究领域：细胞凋亡

细胞色素C(Cyt C)是重要的线粒体蛋白质，位于线粒体膜内叶。在细胞核中编码为apo-细胞色素C。细胞色素C结合在膜上，或作为能量传递膜中的可溶性金属蛋白，存在于细菌、古菌和质体中。属于IIa类细胞色素，含有五配位血红素中心。

應用

细胞色素C（来自牛心）已用于：

  • 作为检测溶液组分，检测人骨肉瘤和人肝癌细胞线粒体中呼吸链复合体III和IV的活性
  • 测定人主动脉内皮细胞线粒体中细胞色素C氧化酶的活性
  • 作为细胞色素C氧化酶反应液组分，用于小鼠肾组织中细胞色素C氧化酶的原位染色

生化／生理作用

在细胞内，细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子转运体。它在动植物的细胞氧化过程中发挥重要作用。它通常被视作呼吸作用的通用催化剂，可在呼吸底物与氧气之间形成必要的电子桥。
细胞色素C(Cyt C)在caspase依赖性内源性凋亡途径参与激活caspase，通过凋亡启动细胞程序性死亡。具有脂结合活性，与心磷脂的相互作用与超氧化酶活性有关。细胞色素C在细胞色素C血红素裂解酶的帮助下结合血红素 ，释放到线粒体膜间隙中。
细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子载体。它在动植物的细胞氧化过程中发挥重要作用。通常视作是呼吸链中的通用连接，它在可呼吸底物与氧气之间形成必要的电子桥。

準備報告

醋酸制备，不需要TCA。

其他說明

想要了解更多细胞色素c和电子传递的信息，请访问www.sigma-aldrich.com/enzymeexplorer.

應用

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儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBZ0555
SLBM4029V
SLBB6437V
031M7022
031M7022V

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Increasing cGMP-dependent protein kinase I activity attenuates cisplatin-induced kidney injury through protection of mitochondria function
Hasiyeti M, et al.
American Journal of Physiology: Renal Physiology (2013)
Stéphane T Gabilly et al.
Frontiers in plant science, 8, 1313-1313 (2017-08-12)
Cytochromes c are hemoproteins, with the prosthetic group covalently linked to the apoprotein, which function as electron carriers. A class of cytochromes c is defined by a CXXCH heme-binding motif where the cysteines form thioether bonds with the vinyl groups
Yong-Ling P Ow et al.
Nature reviews. Molecular cell biology, 9(7), 532-542 (2008-06-24)
Cytochrome c is primarily known for its function in the mitochondria as a key participant in the life-supporting function of ATP synthesis. However, when a cell receives an apoptotic stimulus, cytochrome c is released into the cytosol and triggers programmed
C Garrido et al.
Cell death and differentiation, 13(9), 1423-1433 (2006-05-06)
In healthy cells, cytochrome c (Cyt c) is located in the mitochondrial intermembrane/intercristae spaces, where it functions as an electron shuttle in the respiratory chain and interacts with cardiolipin (CL). Several proapoptotic stimuli induce the permeabilization of the outer membrane
Chu-Chiao Wu et al.
Nature communications, 7, 13565-13565 (2016-11-25)
According to dogma, initiator caspases are activated through proximity-induced homodimerization, but some studies infer that during apoptosis caspase-9 may instead form a holoenzyme with the Apaf-1 apoptosome. Using several biochemical approaches, including a novel site-specific crosslinking technique, we provide the
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Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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