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MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

别名:

Mitochondria Purification Kit

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About This Item

EC號碼:
232-650-8
分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

200

用途

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

儲存期限

2 yr

技術

fractionation: suitable

運輸包裝

dry ice

儲存溫度

−20°C

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應用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分，并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于：
  • 分离线粒体和胞浆组分，用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体，检测线粒体跨膜电位

特點和優勢

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂，即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书

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象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

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5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
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Proceedings of the National Academy of Sciences of the United States of America, 100(6), 3065-3070 (2003-03-13)
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