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Merck
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文件

CS0760

Sigma-Aldrich

线粒体分离物染色试剂盒

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

EC號碼:
217-896-6
分類程式碼代碼:
12352207
NACRES:
NA.32

品質等級

200

用途

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

包裝

pkg of 1 kit

儲存條件

dry at room temperature

技術

flow cytometry: suitable
protein staining: suitable

螢光

λex 490 nm; λem 590 nm (JC-1 dye)

應用

cell analysis
detection

檢測方法

fluorometric

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

應用

独立的线粒体染色试剂盒已被用于评估线粒体内膜的电位。

特點和優勢

  • 简单快捷的方法，可用于对线粒体分离产物进行染色和检测线粒体完整性
  • 包含检测线粒体内膜电化学电位改变所需的全部试剂
  • 包含能够穿透线粒体膜的抗菌性缬氨霉素，因此能够消除线粒体的电化学电位。缬氨霉素可用做对照试剂，来避免JC-1的聚集。

原則

本试剂盒基于JC-1染料的线粒体染色法。在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何导致线粒体膜电位消失的事件（如细胞凋亡）都会导致JC-1染料在线粒体内无法聚集。可通过荧光计或荧光显微镜来检测这一染料。

分析報告

本试剂盒用于检测线粒体的完整性，以及线粒体膜电位的消失事件。

仅试剂盒组分

产品编号
说明
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

象形圖

Skull and crossbones
GHS06

訊號詞

Danger

危險聲明

H300 + H310

危險分類

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse
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Neuroprotective efficacy of mitochondrial antioxidant MitoQ in suppressing peroxynitrite-mediated mitochondrial dysfunction inflicted by lead toxicity in the rat brain
Maiti AK, et al.
Neurotoxicity Research, 31(3), 358-372 (2017)
Subcellular fractionation of mitochondria.
Rice, J.E., and Lindsay, J.G. et al.
Subcellular Fractionation, A Practical Approach, 107-142 (1997)
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