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種類
Type II
品質等級
形狀
lyophilized powder
比活性
≥40 units/mg protein
分子量
25 kDa
成份
protein, ≥85%
顏色
white to off-white
UniProt登錄號
應用
diagnostic assay manufacturing
儲存溫度
−20°C
基因資訊
cow ... CTRB1(618826)
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應用
sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系,使用固定化金属离子亲和层析(IMAC)和固定化金属离子亲和毛细管电泳(IMACE)。
生化/生理作用
α-胰凝乳蛋白酶是丝氨酸肽酶,并且具有通过二硫键连接的三条多肽链(A 链-13 残基,B 链-131 残基和 C 链-97 残基)中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐(DFP)、甲苯磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。
單位定義
一个单元将在 pH 7.8,25℃下,每分钟水解 1.0 μmole BTEE。
準備報告
以 3×结晶的胰凝乳蛋白酶原为原料生产
分析報告
通过 E1%/280 确定的蛋白质
其他說明
有关胰凝乳蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer
基底
产品编号
说明
价格
應用
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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