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Merck

C3888

Sigma-Aldrich

羧肽酶 Y 来源于面包酵母(酿酒酵母

lyophilized powder, ≥50 units/mg protein

别名:

丝氨酸羧肽酶, 肽基-L-氨基酸水解酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

等級

Proteomics Grade

品質等級

形狀

lyophilized powder

比活性

≥50 units/mg protein

分子量

61 kDa

成份

Protein, ≥75% E1%/280

運輸包裝

wet ice

儲存溫度

−20°C

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應用

羧肽酶Y具有广泛的特异性并且在尿素中稳定。因此,该酶与其他羧肽酶不同,可以用于序列分析。由于其酰胺酶作用,该酶可用于具有酰胺化COOH-末端基团的肽,例如催产素和加压素的序列分析。

生化/生理作用

糖蛋白的分子量约为61,000,氮含量为12.74%。它是一个含有442个残基的单条多肽链,在碳水化合物部分含有16个葡糖胺残基。赖氨酸位于NH2末端,-Asp-Ser-Thr-Leu位COOH末端序列。该酶的主要作用是从多肽链上去除COOH末端残基。它可用作液泡标记酶,用于蛋白质转运和定位研究。

包裝

包装尺寸基于蛋白质含量

單位定義

基于EM/230 = 191.5,在25°C,pH 6.75的条件下,一个单位每分钟可将1.0 mole μN-CBZ-Phe-Ala水解为N-CBZ-L-苯丙氨酸和L-丙氨酸。

外觀

含有柠檬酸的冻干粉末。

分析報告

可能存在酰胺酶和酯酶活性

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Caroline A Magalhães et al.
Memorias do Instituto Oswaldo Cruz, 106(2), 146-152 (2011-05-04)
Typical and atypical enteropathogenic Escherichia coli (EPEC) are considered important bacterial causes of diarrhoea. Considering the repertoire of virulence genes, atypical EPEC (aEPEC) is a heterogeneous group, harbouring genes that are found in other diarrheagenic E. coli pathotypes, such as
H R Stennicke et al.
Biochemistry, 35(22), 7131-7141 (1996-06-04)
The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as
L Ballou et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(9), 3368-3372 (1990-05-01)
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae mnn9 mutant bulk mannoprotein. The results support the view that the mnn
Johanna Rankenberg et al.
Experimental eye research, 210, 108704-108704 (2021-07-25)
Advanced glycation end products (AGEs) accumulate with age in human lens capsules. AGEs in lens capsules potentiate the transforming growth factor beta-2-mediated mesenchymal transition of lens epithelial cells, which suggests that they play a role in posterior capsule opacification after
Václava Bauerová et al.
Canadian journal of microbiology, 58(5), 678-681 (2012-04-17)
Vacuoles play an important role in the physiology of pathogenic Candida spp. However, information on Candida albicans vacuolar enzymes, their properties, and regulation is scarce. Expression of the genes APR1 and CPY1 encoding vacuolar aspartic protease and serine carboxypeptidase, respectively

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