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Merck
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C3556

Sigma-Aldrich

过氧化氢酶 来源于人类红细胞

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

别名:

H2O2：H2O2 氧化还原酶

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About This Item

CAS号:
9001-05-2
EC 号:
1.11.1.6 (BRENDA, IUBMB)
EC號碼:
232-577-1
MDL號碼:
MFCD00081483
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

human erythrocytes

品質等級

200

化驗

≥90% (SDS-PAGE)

形狀

buffered aqueous solution

比活性

≥30,000 units/mg protein

分子量

tetramer ~250 kDa

濃度

≤10 mg/mL

技術

cell based assay: suitable

UniProt登錄號

P04040

運輸包裝

wet ice

儲存溫度

−20°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI 密鑰

NUVBSKCKDOMJSU-UHFFFAOYSA-N

基因資訊

human ... CAT(847)

一般說明

研究领域：细胞信号传导

人过氧化氢酶是单功能含血红素过氧化氢酶中的一种。作为细胞内酶，它在肝脏、红细胞和肾脏中浓度较高。过氧化氢酶是同源四聚体蛋白质，由氨基酸残基、血红素基团（铁III原卟啉IX）和磷酸酰胺腺嘌呤二核苷酸（NADPH）分子组成。它是大多数需氧生物中普遍存在的酶。过氧化氢酶（CAT）基因位于人染色体11p13。

應用

来自人类红细胞的过氧化氢酶可用于：

  • 防止还原细胞色素c被H2O2再氧化，同时用细胞色素C测量超氧化物的产生
  • 作为随机光学重建显微镜（STORM）成像人类皮肤成纤维细胞成像缓冲液的组分
  • 作为单分子成像的球状混合物的组分

生化／生理作用

过氧化氢酶缺乏和氧化应激与白癜风、高血压、阿尔茨海默症、糖尿病和脱氢酶血症有关。过氧化氢酶在癌症中表现出两种作用，在慢性淋巴细胞白血病、胶质瘤、黑色素瘤和胃癌中表达水平较高，在急性髓系白血病、胰腺癌、肺癌、前列腺癌和皮肤癌(非黑色素瘤)中表达水平较低。
过氧化氢酶可激活过氧化氢（一种活性氧）分解成水和氧。它起到天然抗氧化剂的作用，可保护细胞免受蛋白质、脂质和核酸的氧化损伤。过氧化氢酶也被用于研究活性氧在基因表达和凋亡中的作用。

單位定義

在pH7.0,25℃条件下，一个单位每分钟可分解1.0 μmole的H2O2 ，H2O2的浓度从从10.3降至9.2mM（通过A240的降低速率进行测定）。

外觀

溶于50mM Tris（pH 8.0）溶液中

儲存和穩定性

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

分析報告

双缩脲法测蛋白

抑制劑

产品编号
说明
价格

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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