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CAT100

Sigma-Aldrich

过氧化氢酶检测试剂盒

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

别名:

Catalase Activity Detection Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

200

用途

sufficient for ≥100 tests enzymatic

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

human ... CAT(847)

相关类别

一般說明

过氧化氢酶活性检测试剂盒包含研究多种组织和亚细胞组织中过氧化氢酶所需的所有组分。

應用

适用于比色法和紫外分光光度法检测

生化／生理作用

过氧化氢酶是一种普遍存在的抗氧化酶，其功能是催化过氧化氢(H2O2) 的分解，生成水和氧气。过氧化氢在真核细胞内以多种氧化酶和超氧化歧化酶的副产物形式存在。过氧化氢在细胞内的聚集会引发细胞内DNA，蛋白质和脂质的氧化，从而导致基因突变和细胞死亡。过氧化氢酶可去除细胞内的H2O2，保护活细胞免受氧化损伤，过氧化氢酶在氧化胁迫相关疾病中的作用已得到了广泛研究。

特點和優勢

  • 用于检测过氧化氢酶活性 - 可能用于各种不同组织和细胞
  • 简单，优化的实验步骤 - 简单的比色法检测过氧化氢酶体富集和过氧化氢酶活性

適合性

适于研究各种组织和亚细胞器中的过氧化氢酶活性。

原則

该测定方法旨在测定过氧化氢酶作用后残留的过氧化氢底物。首先，过氧化氢在过氧化氢酶作用下转化为水和氧气（过氧化氢酶途径），然后通过叠氮化钠终止这一酶促反应。随后通过比色法，分析反应混合物等分试样中余下的过氧化氢量。10 比色法使用取代苯酚（3,5-二氯-2-羟基苯磺酸），在存在过氧化氢和辣根过氧化物酶（HRP）的条件下，通过氧化作用偶联至4-氨基安替比林中，生成吸收波长520 nm的红色醌亚胺染料（N-（4-安替比林基）-3-氯-5-磺酸酯对苯醌单亚胺）。

單位定義

在pH 7.0，25℃，过氧化氢底物浓度50mM条件下，一单位过氧化氢酶每分钟可以分解 1μmol 过氧化氢生成氧气和水。

準備報告

使用超纯水配置所有溶液。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
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  • 323381Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O化学品安全说明书

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书(COA)

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M B Hampton et al.
FEBS letters, 414(3), 552-556 (1997-10-10)
The induction of apoptosis in Jurkat T-lymphocytes with 50 microM hydrogen peroxide was associated with caspase activation. Caspase activity was first detected 3 h after treatment, and the morphological features of apoptosis were apparent by 6 h. At higher concentrations
M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
S Tada-Oikawa et al.
FEBS letters, 442(1), 65-69 (1999-01-29)
Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
M Ding et al.
Journal of cell science, 113 ( Pt 13), 2409-2419 (2000-06-15)
The intracellular protozoan parasite Toxoplasma gondii, like all members of the phylum Apicomplexa, is known to possess many organelles: in addition to mitochondria and the compartments of the secretory pathway, there is a reduced chloroplast (the apicoplast) and the phylum-specific
A J Kowaltowski et al.
FEBS letters, 473(2), 177-182 (2000-05-17)
The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
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