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product name
用于膜的BCIP®/NBT-紫色液体底物系统, ready to use alkaline phosphatase substrate
品質等級
形狀
liquid
儲存溫度
2-8°C
應用
BCIP®/NBT-紫色液体膜底物系统已被用作碱性磷酸酶的底物
- 用于通过免疫印迹法检测二抗
- 用于ELISPOT测定分析
- 用于斑点印迹分析
- 用于免疫印迹分析，以可视化硝酸纤维素膜上的蛋白条带
生化／生理作用
5-溴-4-氯-3-吲哚基磷酸酯 (BCIP)/硝基蓝四唑（NBT）/硝基蓝四唑鎓(NBT)是用于检测碱性磷酸酶(AP)的显色底物系统。AP水解BCIP形成中间体无色靛蓝化合物，并进一步被NBT氧化形成5,5′-二溴-4,4′-二氯靛蓝(BCI)。其中，NBT被还原为不溶的紫色双甲双胍(DF)，形成NBT-DF。NBT-DF和BCI的混合物形成深紫色沉淀。AP可水解多种BCIP分子，因此成为了一种高灵敏度方法。
注意
在2-8 °C稳定至少一年。
外觀
即用型。
法律資訊
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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