A3687
抗 兔 IgG（全分子）-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous glycerol solution
别名:
Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody
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About This Item
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生物源
goat
品質等級
共軛
alkaline phosphatase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous glycerol solution
物種活性
rabbit
技術
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
IgG是糖蛋白抗体，可调节几种免疫反应。针对靶蛋白的兔IgG通常在各种研究应用中用作初级抗体。因此，与可检测底物偶联的次级抗兔IgG是分析靶蛋白的有用工具。山羊抗兔IgG（全分子）-碱性磷酸酶抗体结合所有兔Igs。
免疫原
纯化兔 IgG 作为免疫原
應用
使用碱性磷酸酶缀合的山羊抗兔IgG作为二抗（用5%吐温/PBS按1:2000稀释），采用ELISA法分析小鼠视网膜组织中存在的低氧探针结合蛋白的水平。使用的底物是4-硝基苯基磷酸二钠盐六水合物（Sigma）。
山羊抗兔IgG（全分子）-碱性磷酸酶抗体可用于免疫印迹和ELISA。该抗体也可用于免疫组化（1:50，使用福尔马林固定、石蜡包埋切片）。
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用，使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液（Ph 9.6）中以1：5000稀释的二次稀释剂。
外觀
在含有1mM MgCl 2，10mM甘氨酸，1％牛血清白蛋白，50％甘油和15mM叠氮化钠的0.05M Tris缓冲液（pH 8.0）中的溶液。
免責聲明
除非我们的产品目录或产品随附的其他公司文件中另有说明，否则我们的产品仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
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