B20222

Collagenase - Hyaluronidase blend

Tissue dissociation blend, lyophilized powder, suitable for cell culture

• 分類程式碼代碼: 12352204
• NACRES: NA.54

属性

• 品質等級: 200
• 形狀: lyophilized powder
• 比活性: (Collagenase activity : 1-5 units/ml in 10 ml HBSS (#H6648; FALGPA assay)); (Hyaluronidase activity : 80-400 units/ml in 10 ml HBSS (#H6648; Hyaluronidase assay))
• 濃度: 1 mg/mL (Collagenase)
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: -10 to -25°C

说明

一般說明

The Collagenase-Hyaluronidase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Hyaluronidase. Hyaluronidase is a hyaluronic acid-metabolizing enzyme, who degrades Hyaluronic Acid. Hyaluronic Acid increase tisue and membranes permeability and is one of the main components of the extracellular matrix, contribuing significantly to cell proliferation and migration. Combination of Collagenase and Hyaluronidase is usually used for the isolation of cells from the extracellular matrix.

生化／生理作用

Collagenase: Effective release of cells from tissue requires the action of collagenase enzymes. Collagenase is activated by four-gram atom calcium (Ca2+) per mole enzyme. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction varies from 15 minutes to several hours and yields satisfactory cell dissociation without causing too much cell death. Hyaluronidase: degrades hyaluronan and randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

準備報告

For a working concentration of Collagenase of 1 mg/ml and an enzymatic activity of Hyaluronidase 100 units/ml, reconstitute the lyophilized powder with 10 ml of Hank’s Balanced Salt solution with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate (#H6648)

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable