跳转至内容
Merck
所有图片(1)

主要文件

93289

Sigma-Aldrich

TRI试剂®

别名:

TRI试剂® RNA 分离试剂

登录查看公司和协议定价
所有图片(1)

About This Item

MDL號碼:
MFCD00213058
分類程式碼代碼:
12352200
NACRES:
NA.52

形狀

liquid

用途

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue

雜質

phenol

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

TRI reagent®也称为TRIzol，是苯酚和异硫氰酸胍的单相溶液。它可能同时溶解生物材料并使蛋白质变性。这一产品广泛用于RNA脱蛋白。由Chomczynski开发的这种改进版的一步总RNA分离试剂可以快速、经济、有效地分离来自不同生物样品的总RNA。该产品是纯净的（A260/A280 ~1.8）并且可以直接用于Northern印迹。还可同时分离DNA、RNA和蛋白质。1 mL足以用于提取100 mg组织或107个细胞。

應用

TRI Reagent®已用于从各类细胞或组织中分离RNA。这一产品还可以用于处理组织、单层培养细胞或细胞团块。
TRI试剂是对由Chomczynski开发的一步法总RNA分离试剂进行改良的一种版本。基于该试剂的RNA分离方法已被广泛用于和验证用于RNA应用。它是对来自人、动物、植物、酵母、细菌和病毒来源样本进行总RNA或RNA、DNA和蛋白同时分离的一种理想的快速、经济和高效的方法。

特點和優勢

  • 可轻松扩展的RNA分离
  • 可适用于多种来源：人、植物、酵母、细菌或病毒
  • 相对于传统的硫氰酸胍/氯化铯具有更高的产量

法律資訊

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

標靶器官

Nervous system,Kidney,Liver,Skin

安全危害

EUH032

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 2

閃點（°F）

174.2 °F - closed cup

閃點（°C）

79 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCH1222
BCCF6176
BCCD4264
BCCC4846
BCCB4526

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Hybrid amniotic membrane dressing with green silver nanoparticles as bioengineered skin for wounds and burns: a pilot studies
Ramesh B, et al.
Journal of biotechnology and biomaterials, 7(272), 2-2 (2017)
Expression of adenosine receptors and vegf during angiogenesis and its inhibition by pentoxifylline?a study using zebrafish model
Nathan J, et al.
Biomedicine and Pharmacotherapy, 84(6), 1406-1418 (2016)
Hao Zhang et al.
Molecular medicine reports, 22(2), 915-925 (2020-07-07)
Dilated cardiomyopathy (DCM) is a primary cardiomyopathy with high mortality. The aim of the present study was to identify the related genes in DCM. The four expression profiles (GSE17800, GSE21610, GSE42955 and GSE79962) downloaded from the Gene Expression Omnibus (GEO)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
Wang Xing et al.
Journal of receptor and signal transduction research, 41(5), 476-487 (2020-10-16)
MicroRNA (miRNA) is known to be involved in the pathological process of congenital heart disease (CHD), and nodal modulator1 (NOMO1) is a critical determinant of heart formation. The present study aims to discover the effect of miR-33a-5p and NOMO1 on
查看所有相关文献

商品

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

TRI Reagent® Protocol

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门