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品質等級
化驗
≥98.0% (HPLC)
形狀
solid
螢光
λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)
適合性
suitable for fluorescence
儲存溫度
2-8°C
SMILES 字串
COC1(OC=C(C1=O)c2ccccc2)c3ccccc3
InChI
1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3
InChI 密鑰
BLWINLJDTOJSRU-UHFFFAOYSA-N
應用
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.
其他說明
Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE
訊號詞
Warning
危險分類
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
Pablo Castro-Hartmann et al.
Electrophoresis, 25(15), 2501-2505 (2004-08-10)
The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with
F J Alba et al.
Electrophoresis, 18(11), 1960-1966 (1998-01-07)
We have studied the light emission efficiency of proteins labeled with different fluorescent dyes chemically excited by the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 reaction. Using this peroxyoxalate chemiluminescence system, the best results were obtained with proteins covalently labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF). Blotted proteins
M Hengstschläger et al.
Cytometry, 14(1), 39-45 (1993-01-01)
Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for diagnosis. Although of great interest, its regulation during cell cycle and differentiation is difficult to study, as current techniques for isolation of cells in different
J C Stockert et al.
Blood cells, 19(2), 423-430 (1993-01-01)
The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this work, we describe the application of
S. Stein
Pept. Neurobiol., 9-9 (1977)
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