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Merck

62971

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

powder (crystalline), 70000-140000 U/mg

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.22

生物源

chicken egg white

品質等級

形狀

powder (crystalline)

比活性

70000-140000 U/mg

分子量

single-chain 14.3 kDa
Mr ~14600

技術

cell based assay: suitable

適合性

suitable for cell lysis

UniProt登錄號

應用

cell analysis

儲存溫度

2-8°C

基因資訊

chicken ... LYZ(396218)

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應用

酶可分解细菌的细胞壁;用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。

單位定義

在pH 7.0和25℃条件下,1 U对应于每分钟使450 nm处的吸光度降低0.001的酶量(藤黄微球菌,ATCC 4698,作为底物)

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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