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一般說明
按照 C.H. Fiske 和 Y. Subbarow(J.Biol.Chem. 66,375 (1925))的方法配制的 1-氨基-2-萘酚-4-磺酸、亚硫酸钠和亚硫酸氢钠
應用
The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 4 Oral - Eye Dam. 1
安全危害
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
The colorimetric determination of phosphorus
The Journal of Biological Chemistry, 66, 375-375 (1925)
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