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EAGLETMMRO

Roche

EagleTaq 通用预混液 (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

用途

sufficient for 100 reactions
 sufficient for 500 reactions
 sufficient for 5000 reactions

品質等級

100

特點

dNTPs included
hotstart

製造商／商標名

Roche

包裝

pkg of 100 x 20 μL reactions (07260288190)
pkg of 500 x 20 μL reactions (07260296190)
pkg of 5000 x 20 μL reactions (07249926190)

技術

RT-qPCR: suitable
qPCR: suitable

輸入

purified DNA

檢測方法

probe-based

相关类别

一般說明

2× 浓缩的即用型热启动预混液，用于 qPCR 和 qRT-PCR，使用实时 PCR 仪器上的水解探针检测格式。

EagleTaq Universal Master Mix(ROX)是一种可直接使用的2×浓缩PCR预混液，包含执行定量、实时PCR水解反应探针所需的所有试剂（除了引物、探针和模板）。它包含一种特殊的ROX参考染料，适用于所有需要ROX参考染料进行定量分析的实时仪器。
EagleTaq Universal Master Mix (ROX)含有dUTP，因此它可以与尿嘧啶DNA糖基酶(UNG)一起使用，以防止由于携带污染而产生的假阳性。
热启动特性允许在环境温度下进行反应设置。
有了这种稳定的试剂，任何PCR方案只需要最低程度的优化。
EagleTaq Universal Master Mix(ROX)是一种可直接使用的2×浓缩聚合酶链反应(PCR)预混液，包含执行定量、实时PCR (qPCR)和定量逆转录PCR (qRT-PCR)水解反应探针所需的所有试剂（除了引物、探针和模板）。

應用

EagleTaq Universal Master Mix (ROX)已用于定量、实时聚合酶链反应(qPCR)和定量反转录PCR (qRT-PCR)。

特點和優勢

  • EagleTaq Universal Master Mix (ROX)包含一种特殊的ROX参考染料，适用于所有需要ROX参考染料进行定量分析的实时仪器。
  • 它含有dUTP，因此它可以与尿嘧啶DNA糖基酶(UNG)一起使用，以防止由于携带污染而产生的假阳性。
  • 热启动特性允许在环境温度下进行反应设置。
  • 有了这种稳定的试剂，任何PCR方案只需要最低程度的优化。

成分

含有 EagleTaq DNA 聚合酶和 dATP、dCTP、dGTP、dUTP、MgCl2 和 ROX 的反应缓冲液。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

does not flash

閃點（°C）

does not flash

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Hiroto Suwa et al.
Biochemistry and biophysics reports, 8, 62-67 (2016-08-12)
Retinoic acid (RA) is a metabolite of vitamin A and has important roles in development, differentiation, and reproduction. Activin has been shown to regulate the RA pathway and affect granulosa cell (GC) proliferation, suggesting that RA is important for early
Ai Nakaoka et al.
Oncology reports, 45(4) (2021-03-03)
The precise mechanism of intercellular communication between cancer cells following radiation exposure is unclear. Exosomes are membrane‑enclosed small vesicles comprising lipid bilayers and are mediators of intercellular communication that transport a variety of intracellular components, including microRNAs (miRNAs or miRs).
James Meixiong et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(21), 10525-10530 (2019-05-10)
Patients suffering from cholestasis, the slowing or stoppage of bile flow, commonly report experiencing an intense, chronic itch. Numerous pruritogens are up-regulated in cholestatic patient sera, including bile acids (BAs). Acute injection of BAs results in itch in both mice

商品

PCR Master Mix

A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.

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Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

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