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Merck

11412272001

Roche

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

别名:

hTGF

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About This Item

分類程式碼代碼:
12352207

生物源

human

品質等級

重組細胞

expressed in CHO cells

化驗

>95% (SDS-PAGE)

形狀

solution

效力

<0.1 ng/mL EC50

分子量

25,000 Da

包裝

pkg of 1 mL (1 μg)

製造商/商標名

Roche

儲存條件

avoid repeated freeze/thaw cycles

雜質

<0.1 EU/μgtested (LAL test)

UniProt登錄號

儲存溫度

−20°C

基因資訊

human ... TGFB1(7040)

一般說明

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

特異性

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

應用

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

生化/生理作用

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

品質

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

序列

Chain Length 2 x 112 AA
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

單位定義

EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

準備報告

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

does not flash

閃點(°C)

does not flash


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Periostin is essential for periodontal ligament remodeling during orthodontic treatment.
Xu HY, et al.
Molecular Medicine Reports, 15(4), 1800-1806 (2017)
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Pan, Hongjie, and Jaroslava Halper
Biochemical and Biophysical Research Communications, 24-30 (2003)
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Hayashi E, et al.
Inflammation and regeneration, 35(5), 244-254 (2015)

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