SCT280

BioTracker^™ Far Red Live Cell Cycle Assay Solution

Human

• 别名: cell cycle dye, cell cycle indicator, cell cycle probe, live cell probe
• 分類程式碼代碼: 41106514
• NACRES: NA.47

属性

• product name: BioTracker^™ Far Red Live Cell Cycle Assay Solution,
• 生物源: human
• 品質等級: 100
• 包裝: vial of 50 tests
• 製造商／商標名: Millipore
• 技術: cell culture | stem cell: suitable; flow cytometry: suitable
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Propidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker^™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells. Use 5 uL of probe solution in 500 uL PBS for each sample. Vial contains enough for 50 tests.The far red probe uses the 633 laser, enabling multiplexing with shorter wavelength probes by freeing the 488 laser for probes that emit in the green range of the spectrum. IMPORTANT APPLICATION NOTES:*Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.*Adjust gates after cells are stained with probe SPECTRAL PROPERTIESExcitation: 633 - 647 nmEmission: 695 LP or 780/60 BP.A filter set for APC-Cy7 is suitable.

特點和優勢

Far red fluorescence probe for analysis of cell cycle by flow cytometry.

標靶描述

The control system of the cell cycle is closely linked to cell proliferation. Cell division in which one cell produces two daughter cells occurs as part of the cell cycle. The cell cycle can be assessed by analyzing cells stained for DNA using flow cytometry, followed by analysis of the proportion of stained nuclei in cell cycle phase. The cell cycle is classified into two major phases: interphase and the mitotic (M) phase. Interphase is composed of G1 phase (preparation for DNA synthesis which occurs prior to cell division), S phase (DNA synthesis and replication), and G2 phase (a single cell with duplicated chromosomes). M phase involves mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. To determine the action of anticancer drugs and others, it is important to analyze the mechanism of cell-cycle checkpoint.

儲存和穩定性

Store BioTracker^™ Far Red Live Cell Cycle Assay Solution at -20 °C, protected from light.

其他說明

Live cell probe for fluorescent cell cycle analysis. Unlike propidium iodide, this probe is membrane permeable and highly specific for DNA, which makes it possible to analyze the cell cycle by simply adding the reagent to a cell suspension.

法律資訊

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS05,GHS09
• 訊號詞: Danger
• 危險聲明: H318,H411
• 防範說明: P273 - P280 - P305 + P351 + P338 - P391 - P501
• 危險分類: Aquatic Chronic 2 - Eye Dam. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3