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Merck
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Key Documents

SCT265

Sigma-Aldrich

BioTracker Green Exosome Membrane Labeling Kit

Human

别名:

exosome labeling kit, exosome membrane probe, green exosome dye, green exosome probe

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About This Item

分類程式碼代碼:
41106514
NACRES:
NB.24

product name

BioTracker Green Exosome Membrane Labeling Kit,

生物源

human

品質等級

包裝

vial of 5 tests (reactions)

製造商/商標名

Millipore

技術

cell culture | stem cell: suitable
flow cytometry: suitable

運輸包裝

2-8°C

儲存溫度

2-8°C

一般說明

The BioTracker exosome membrane labeling probe series can be used for staining of the membranes of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.SCT265 does not cause aggregation and has little effect on the properties of exosomes, permitting accurate observation of exosome dynamics.BioTracker exosome membrane labeling probes overcome issues with existing labeling dyes, including:1) self-particle formation2) an increase in size of the exosomes after labelingThree different fluorescence colors---green (SCT265), red (SCT266), and far red (SCT267)--- enable application in multiplexing experiments, such as observation of exosome localization in organelles.Add 10 uL DMSO to tube of green, red, or far red exosome membrane dye to create stock solution. SPECTRAL PROPERTIES:Ex 488nm/Em 490-540nmReferences:Kawata R, Oda S, Koya Y, Kajiyama H, Yokoi T. 2020. Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production. Toxicology. 443:152544.

特點和優勢

This live cell probe fluoresces in the green spectral range when it binds to the lipid bilayer in exosome membranes.

標靶描述

Exosomes are a form of extracellular vesicle (EV), which can contribute to intercellular communication. To elucidate such communication, labeling techniques based on fluorescent dyes have been developed. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target.

儲存和穩定性

Store BioTracker Green Exosome Membrane Labeling Kit at 2 - 8 °C, protected from light.After resuspending in DMSO, store stock solution at -20 °C, protected from light.

其他說明

This live culture probe for the detection of exosomes labels the lipid bilayers of exosomal membranes, enabling observation of the uptake of exosomes by cells. Exosomes should be purified before labeling.

法律資訊

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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