SCCAR10148

Assay Ready MB49 Mouse Bladder Carcinoma Cell Line

• 别名: MB-49, mouse bladder carcinoma, murine bladder carcinoma
• 分類程式碼代碼: 41106514
• NACRES: NA.81

属性

• 生物源: human
• 品質等級: 100
• 包裝: vial of 1 vial
• 製造商／商標名: Millipore
• 技術: cell culture | stem cell: suitable
• 運輸包裝: liquid nitrogen
• 儲存溫度: −196°C

说明

一般說明

MB49 cells are derived from C57BL/Icrf-a′ mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture (1). Transformed cells transplanted into syngeneic mice were shown to generate carcinomas (1). While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed (2). This abnormality is a frequent early event in human bladder cancer.

A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth (3). MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone, human chorionic gonadotrophin (hCG) (3).

MB49 cells exhibit low to no expression of MHC-Class I and II molecules (4). However, upon exposure to IFN-?, the expressions of MHC Class I and II are significantly upregulated (4).

References:
1. Summerhayes IC, Franks LM (1979) Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro. J Natl Cancer Inst 62(4): 1017 - 1023.
2. Fabris VT, Lodilinsky C, Pampena MB, Belgorosky D, Lanari C, Eiján AM (2012) Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive line MB49-I. Cancer Genet 205(4): 168 - 76.
3. White-Gilbertson S, Davis M, Voelkel-Johnson C, Kasman LM (2016) Sex differences in the MB49 syngeneic, murine model of bladder cancer. Bladder (San Franc) 3(1): PMID: 26998503.
4. Lattime EC, Gomelia LG, McCue PA (1992) Murine bladder carcinoma cells present antigen to BCG-specific CD4+ T-cells. Cancer Res 52(15): 4286 - 90.

特點和優勢

MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone human chorionic gonadotrophin (hCG) [3]. MB49 cells exhibit low to no expression of MHC-Class I and II molecules [4]. However, upon exposure to IFN-?, the expressions of MHC Class I and II are significantly upregulated

標靶描述

MB49 cells are derived from C57BL/Icrf-a mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture [1]. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas [1]. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed [2]. This abnormality is a frequent early event in human bladder cancer. A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth.

儲存和穩定性

MB49 cells should be stored in liquid nitrogen.

其他說明

This product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable