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Merck
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主要文件

NS170

Sigma-Aldrich

Multi Species Phosphorylated Neurofilament ELISA Kit

measures and quantifies pNF-H levels in serum or plasma

别名:

200 kDa neurofilament protein, Phosphorylated Neurofilament heavy polypeptide, pNF-H

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

product name

磷酸化神经丝(pNF-H)夹心ELISA试剂盒, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.

品質等級

物種活性

pig, feline, rat, human, bovine, mouse

製造商/商標名

Chemicon®

assay range

sensitivity: 0.0293-15 ng/mL
(Range of Detection)

sensitivity: 0.0585 ng/mL

技術

ELISA: suitable

NCBI登錄號

UniProt登錄號

應用

research use

檢測方法

colorimetric

運輸包裝

dry ice

應用

用于检测/量化:磷酸化神经丝(pNF-H)
研究类别
神经科学
磷酸化神经丝H(pNF-H)试剂盒是一种夹心酶免疫测定法(EIA),可测量pNF-H。

包裝

96孔

成分

鸡抗-pNF-H多克隆抗体预包被的免疫板:(部件号2006263)。一个预先包被有鸡抗pNF-H多克隆抗体的96孔免疫板,密封在铝箔袋中。

兔抗pNF-H多克隆抗体:(部件号2006919)。一瓶(100 μL)兔抗pNF-H多克隆抗体。

山羊抗兔碱性磷酸酶偶联抗体:(部件号2006917)。一瓶(10 μL)山羊抗兔碱性磷酸酶结合物。

牛pNF-H标准品:(部件号2006918)。一瓶(5.0 ng)牛pNF-H标准品。

20X TBS:(部件号2004913)。一瓶(50 mL)20X TBS。

20% 吐温-20® :(部件号2004915)。一瓶(3 mL)20% 吐温-20。

Elisa检测稀释剂:(部件号2006921)一瓶(30 mL)Elisa检测稀释剂,含有0.05%Kathon作为防腐剂,以即用型形式提供。

pNPP缓冲液:(部件号2006261)。一瓶(12 mL)pNPP缓冲液,与50X p-NPP一起使用,以即用型形式提供。

50X pNPP:(部件号2006262)。一瓶(250 μL)50X pNPP(对硝基苯基磷酸酶)碱性磷酸酶底物。

板盖-两个板盖。

儲存和穩定性

使用前,按照指示将试剂盒组件保持在2°-8°C和-20°C,直至失效日期。

1.清洗缓冲液-稀释后,将清洗液在2°-8°C下保存长达一个月或直至失效日期。

2.吐温-20® - 收到试剂盒后在室温下储存。

3.Elisa试验缓冲液-根据需要等分,以避免重复冻融循环。解冻后,将Elisa分析缓冲液在2°-8°C下保存长达一个月,或直至失效日期。

4. 预先用鸡抗pNF-H多克隆抗体包被的免疫板-未使用的试剂条微孔可放回铝箔袋中,并在2°-8°C下储存长达1个月或直至失效日期。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

免責聲明

仅供研究使用。不用于诊断操作。

象形圖

Health hazardCorrosion

訊號詞

Danger

危險分類

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

標靶器官

Kidney,Liver,Blood

儲存類別代碼

10 - Combustible liquids


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Johannes Brettschneider et al.
Journal of neurology, 256(1), 3-12 (2009-03-10)
Guillain-Barré syndrome (GBS) is an acute inflammatory polyneuropathy affecting the myelin-protein sheathing and the axons themselves to various degrees. Damage to these structures causes biomarkers to be released into the adjacent body fluid compartment. In case of the proximal nerve
Axel Petzold et al.
Journal of immunological methods, 319(1-2), 34-40 (2006-12-05)
Recent studies suggest that the quantification of neurofilament subunits in cerebrospinal fluid (CSF), blood and amniotic fluid may reflect neuroaxonal damage and be of high clinical value. The present study aims to cross-validate two different independently developed ELISA techniques for
R Takano et al.
Neurology, 75(3), 208-216 (2010-07-21)
Loss of aquaporin 4 and glial fibrillary acidic protein (GFAP) with necrosis and demyelination is a prominent pathologic feature of neuromyelitis optica (NMO). However, the clinicopathologic significance of astrocytic damage and its relation with demyelination are unknown. To analyze clinical
J S Pachter et al.
Developmental biology, 103(1), 200-210 (1984-05-01)
The ontogenetic appearance of the individual triplet polypeptides that comprise mammalian neurofilaments was studied in the developing rat optic nerve. Triton-insoluble cytoskeletal preparations from the optic nerves of rats of postnatal ages 1 Day (P1), 6 days (P6), 10 days
Gerry Shaw et al.
Biochemical and biophysical research communications, 336(4), 1268-1277 (2005-09-24)
Several lines of reasoning suggest that the phosphorylated axonal form of the neurofilament subunit NF-H is likely to be released from damaged and diseased neurons in significant amounts. Detection of this protein in serum or CSF might therefore provide information

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