推荐产品
生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
6F11, monoclonal
物種活性
human, mouse
技術
immunocytochemistry: suitable
western blot: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ATE1(11101)
一般說明
ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway. ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins. The arginylation is required for effective ubiquitin mediated protein degradation. ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation. This system disposes of malformed or misdirected proteins and protein fragments. ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized. ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation. Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimer′s disease, amyotrophic lateral sclerosis, and Parkinson′s disease.
免疫原
Recombinant protein corresponding to mouse ATE1.
應用
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-ATE1 Antibody, clone 6F11 is validated for use in Western Blotting and Immunocytochemistry for the detection of ATE1.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected ATE1 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.
標靶描述
~60 kDa observed
外觀
Protein G Purified
Format: Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Marie E Fina et al.
Scientific reports, 11(1), 9376-9376 (2021-05-02)
Regulator of G-protein signaling 7 (RGS7) is predominately present in the nervous system and is essential for neuronal signaling involving G-proteins. Prior studies in cultured cells showed that RGS7 is regulated via proteasomal degradation, however no protein is known to
Junling Wang et al.
Chemistry & biology, 18(1), 121-130 (2011-02-01)
Posttranslational arginylation mediated by arginyl transferase (ATE1) plays an important role in cardiovascular development, cell motility, and regulation of cytoskeleton and metabolic enzymes. This protein modification was discovered decades ago, however, the arginylation reaction and the functioning of ATE1 remained
Iuliia Pavlyk et al.
Traffic (Copenhagen, Denmark), 19(4), 263-272 (2018-02-01)
β-actin plays key roles in cell migration. Our previous work demonstrated that β-actin in migratory non-muscle cells is N-terminally arginylated and that this arginylation is required for normal lamellipodia extension. Here, we examined the function of β-actin arginylation in cell
Chunhua Jiang et al.
Frontiers in cell and developmental biology, 8, 603688-603688 (2021-01-08)
Arginyltransferase 1 (ATE1) is an evolutionary-conserved eukaryotic protein that localizes to the cytosol and nucleus. It is the only known enzyme in metazoans and fungi that catalyzes posttranslational arginylation. Lack of arginylation has been linked to an array of human
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