MAB1285F

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated

clone HLA.ABC.m3, Chemicon^®, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

属性

• 生物源: mouse
• 品質等級: 100
• 共軛: FITC conjugate
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: HLA.ABC.m3, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: flow cytometry: suitable; immunofluorescence: suitable
• 同型: IgG2a
• NCBI登錄號: NM_005514.5
• UniProt登錄號: P01889
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HLA-B(3106)

说明

一般說明

BIOCHEMISTRY: pI = 8.0

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

特異性

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

免疫原

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

應用

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

外觀

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

儲存和穩定性

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable