推荐产品
生物源
mouse
品質等級
化驗
>95% (HPLC and SDS-PAGE)
形狀
liquid
比活性
≥1 x 10(E8) U/mg
製造商／商標名
Chemicon®
濃度
10 μg/mL
技術
cell culture | stem cell: suitable
雜質
<0.1 ng/mg Endotoxin (of LIF)
輸入
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
應用
推荐的QC方案
M1生物测定
1.使用体外半固体琼脂培养物进行M1生物测定，该培养物在1 mL体积的DME（含20%FCS的0.3%琼脂）中含有约100个细胞。
2.加入100 μL样品或mLIF（等渗盐水中5%FCS中的10（E4）单位/mL），一式两份两倍系列稀释至35 mm培养皿。
3.将100μ L 5%FCS的等渗盐水加入到两个对照载玻片上。
4.在37°C、含10%CO2的完全湿润空气中培养7天。
5.对显示分化的菌落数进行评分（注：50单位定义为导致50%菌落分化的活性量）。
有关其他信息，请访问www.esgro-lif.com
LIF由CHEMICON International，Inc.生产，受美国专利号5,187,077、5,427,925、5,443,825、5,750,654 和 6,261,548，欧洲专利号 0285 448 和相关的外国专利的保护，并无法再销售。
M1生物测定
1.使用体外半固体琼脂培养物进行M1生物测定，该培养物在1 mL体积的DME（含20%FCS的0.3%琼脂）中含有约100个细胞。
2.加入100 μL样品或mLIF（等渗盐水中5%FCS中的10（E4）单位/mL），一式两份两倍系列稀释至35 mm培养皿。
3.将100μ L 5%FCS的等渗盐水加入到两个对照载玻片上。
4.在37°C、含10%CO2的完全湿润空气中培养7天。
5.对显示分化的菌落数进行评分（注：50单位定义为导致50%菌落分化的活性量）。
有关其他信息，请访问www.esgro-lif.com
LIF由CHEMICON International，Inc.生产，受美国专利号5,187,077、5,427,925、5,443,825、5,750,654 和 6,261,548，欧洲专利号 0285 448 和相关的外国专利的保护，并无法再销售。
單位定義
比活度：50 个单位定义为在 1 mL 琼脂培养物中诱导 50％ M1 克隆分化所需的小鼠 LIF 量。
分析報告
无菌和微质体测试均为阴性。
比活度：小鼠 LIF 的活性取决于诱导小鼠 M1 髓样白血病细胞分化的能力。在该测定法中，小鼠LIF的最低可检测浓度为 0.5 ng/mL
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Yuanji Lin et al.
Molecular cell, 48(4), 627-640 (2012-10-09)
Signaling via the Akt serine/threonine protein kinase plays critical roles in the self-renewal of embryonic stem cells and their malignant counterpart, embryonal carcinoma cells (ECCs). Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine
Kay Jüngling et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2100-2102 (2003-09-23)
The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous
Tomoko Ishibashi et al.
Neuron, 49(6), 823-832 (2006-03-18)
Myelin, the insulating layers of membrane wrapped around axons by oligodendrocytes, is essential for normal impulse conduction. It forms during late stages of fetal development but continues into early adult life. Myelination correlates with cognitive development and can be regulated
K Kami et al.
Muscle & nerve, 22(11), 1576-1586 (1999-10-08)
Using in situ hybridization histochemistry, we characterized the spatiotemporal gene expression patterns of leukemia inhibitory factor (LIF) and glial cell line-derived neurotrophic factor (GDNF), and their receptor components (LIFR, GFR-alpha1, RET) induced in muscle cells, intramuscular nerves, and motoneurons in
M K Carpenter et al.
Experimental neurology, 158(2), 265-278 (1999-07-23)
The isolation and expansion of human neural progenitor cells have important potential clinical applications, because these cells may be used as graft material in cell therapies to regenerate tissue and/or function in patients with central nervous system (CNS) disorders. This
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