推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
41-1E5, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
應無反應活性
bovine
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG2a
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MMP1(4312)
一般說明
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.MMP-1 (interstitial collagenase, tissue collagenase, fibroblast collagenase) is secreted as a 57/52 kDa zymogen which is proteolytically processed to the 46/42 kDa active forms. This enzyme displays substrate specificity toward type I, II, III, VII, VIII and X collagens and gelatin. MMP-1 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis, osteoarthritis, and cancer cell invasion.
Purified mouse monoclonal antibody. Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
免疫原
Human
a synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
應用
Frozen sections (see application references)
Immunoblotting (1 g/ml)
Paraffin sections (2.5 g/ml, pressure cooker pre-treatment required)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外觀
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
重構
Following initial thaw, aliquot and freeze (-20°C).
其他說明
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta.219, 1.
Woessner, J.F. 1991. FASEB5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
Stetler-Stevenson, W.G., et al. 1993. FASEB7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta.219, 1.
Woessner, J.F. 1991. FASEB5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
To prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
法律資訊
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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