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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
SL-1 IID4, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2b
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... MMP3(4314)
一般說明
MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.
特異性
MAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.
CELLULAR LOCALIZATION:
Cytoplasmic
CELLULAR LOCALIZATION:
Cytoplasmic
免疫原
APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1
應用
Anti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).
Immunofluorescence
Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.
Western blotting: 0.5-1.0 μg/ml for 2hrs at RT
Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.
Optimal working dilutions and protocols must be determined by end user.
Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.
Western blotting: 0.5-1.0 μg/ml for 2hrs at RT
Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.
Optimal working dilutions and protocols must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
外觀
Format: Purified
Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
儲存和穩定性
Antibody with sodium azide is stable for 12 months when stored at 2-8°C.
分析報告
Control
POSTIVE CONTROL:
Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.
POSTIVE CONTROL:
Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
G Galazka et al.
Biochemistry, 35(34), 11221-11227 (1996-08-27)
Matrix metalloproteinases (MMPs) can be activated in vitro by multiple mechanisms such as treatment with proteases, organomercurials, oxidants, and detergents. The proposed cysteine switch model for activation suggests that these multiple methods for activation cause the dissociation of the single
MMP-2, -3 and -9 levels and activity are not related to Abeta load in the frontal cortex in Alzheimer's disease.
S Baig,P G Kehoe,S Love
Neuropathology and Applied Neurobiology null
Stromelysin-1/matrix metalloproteinase-3 (MMP-3) expression accounts for invasive properties of human astrocytoma cell lines.
Javier Mercapide, Ricardo Lopez De Cicco, Javier S Castresana, Andres J P Klein-Szanto
International Journal of Cancer. Journal International Du Cancer null
Adel Ghoul et al.
Folia histochemica et cytobiologica, 55(2), 62-73 (2017-06-22)
Elevated plasma homocysteine (Hcy) levels have been associated with several tissue injuries including heart and liver fibrosis. In these diseases, hyperhomocysteinemia (Hhcy) plays a major role in modulating the alteration of the balance between matrix metalloproteinases (MMP) and tissue inhibitors
Asha Shahed et al.
Molecular reproduction and development, 75(9), 1433-1440 (2008-01-24)
Siberian hamsters adapt to seasonal changes by reducing their reproductive function during short days (SD). SD exposure reduces uterine mass and reproductive capacity, but underlying cellular mechanisms remain unknown. Because matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are important
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